HRZZ MOBILITY IN TORINO – part V.

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In addition to in vitro grapevine cultivation techniques, Emanuel has been involved in numerous other types of scientific activities and has strengthened his collaboration with the exceptional team from Turin, led by Giorgio Gambino, with whom the collaboration will certainly continue. As an inexperienced doctoral student at the beginning of his scientific career, this opportunity that the Croatian Science Foundation offers to all assistants in Croatia represents a unique opportunity to expand his knowledge, but also to develop valuable acquaintances and collaborations that are essential for personal and professional development.

A message to all young doctoral students/assistants: If you have the opportunity to apply for a short-term mobility offered by the Croatian Science Foundation, take advantage of it because this is a rare opportunity for significant progress and scientific maturation! Ci vediamo 🙂

HRZZ MOBILITY IN TORINO – part IV.

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Join us for an informative seminar on the topic of the interaction of grapevines with viruses and mycorrhizal fungi, where Emanuel will present the results of the MycoGrape project as well as his activity at the Institute for Sustainable Plant Protection, where he learns the basics of in vitro cultivation of virus-free grapevines for use in future research in grapevine interactions with pathogenic and beneficial microorganisms.

Access link: https://events.teams.microsoft.com/event/784a4f53-edb0-489f-8733-83ff6813c026@34c64e9f-d27f-4edd-a1f0-1397f0c84f94

HRZZ MOBILITY IN TORINO – part III.

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In addition to the skills acquired in isolating explants and planting them on nutrient media, Emanuel also acquired other skills necessary for in vitro cultivation of grapevines. Thus, the procedure for maintaining and propagating grapevines as well as their gradual acclimatization to outdoor conditions was learned.

HRZZ MOBILITY IN TORINO – part II.

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At the Institute for Sustainable Plant Protection, Emanuel is actively learning parts of the grapevine tissue culture protocol. Starting with familiarization with the protocols, laboratory equipment and the preparation of different types of nutrient media for stimulating the development of callus and embryos from the anthers and ovaries from grapevine inflorescence. In Turin, the correct method of sampling, storing and sterilizing of grapevine inflorescences has also been learned. From such inflorescences, a large number of anthers and ovaries will be isolated on a nutrient medium for callus induction.

Somatic embryogenesis is the process of developing embryos from cells that are not produced by the fusion of gametes, but have been differentiated into a somatic cells. In this case, in vitro development of an embryo from anthers/ovaries involves the dedifferentiation of the cell into a totipotent cell that gradually differentiates into callus tissue, and then an embryo from which a new plant develops.

HRZZ MOBILITY IN TORINO – part I.

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The Croatian Science Foundation (HRZZ) has funded a five-week outgoing mobility of doctoral student Emanuel at the Institute for Sustainable Plant Protection in Turin. During this period, Emanuel will work in the Functional Genomics and Ecophysiology Laboratory, led by Dr. sc. Giorgio Gambino. The aim of the mobility will be to familiarize himself with the process of somatic embryogenesis and master the basic techniques used in in vitro tissue culture.

Article published in Journal of Plant Physiology

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Another scientific paper has emerged from the collaboration of partners on the project, titled: “Tripartite interactions between grapevine, viruses, and arbuscular mycorrhizal fungi provide insights into modulation of oxidative stress responses.”

Highlights

  • AMF are integral part of ROS homeostasis in grapevine coping with virus stress.
  • AMF reduced activity of ascorbate-glutathion cycle implying reduced ROS production.
  • In the later phase of AM establishment, AMF intensifed H2O2 reduction by GPOD.
  • STS1 and EDS1 relative expressions confirm lower oxidative stress in AMF plants.
  • Virus infected grapevine shows reduced oxidative stress due to AMF symbiosis.

Article can be accessed here.

Published article in Phytobiomes Journal

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A paper titled ‘Arbuscular mycorrhizal fungi modify temporal virus accumulation and distribution in different grapevine tissues’ has been published, resulting from the collaborative efforts of MYCO GRAPE project contributors. The paper explores the effects of mycorrhizal fungi on changes in virus concentration in various grapevine tissues over a one-year period. The paper’s abstract is provided below, and the full text can be accessed here.

ICOM 12 Conference

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At the 12th International Mycorrhizal Conference (ICOM12), which took place from Sunday 4 to Friday 9 August 2024 in Manchester, Dr. sc. Tomislav Radić and Emanuel Gaši presented the new results of the MycoGrape project with poster presentations. In a pleasant atmosphere with the most influential scientists of the mycorrhizal community, the latest knowledge and the future of mycorrhizal research were exchanged under the title of the congress “Bridging gaps in form, function and diversity”. For more information about the congress, click here, and you can find a summary of all presented research below in the book of abstracts. Also, the posters of Dr. Radić and PhD student Emanuel are available in pdf format below.

ICOM12-Abstract-E-Book-1Preuzmi
Poster_Icom12_RadicPreuzmi
ICOM12_Gasi-2Preuzmi

Training in Ljubljana

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Emanuel Gaši, PhD student from the Institute for Adriatic Crops and Karst Reclamation, Split, Croatia, has successfully attended a one – week training period on the data analysis and data interpretation, using open-source statistical analysis software R-Studio. The training took place from 20th May to 24th May 2024 at the Department of Biology, Biotechnical Faculty, University of Ljubljana, with guidance from professor Matevž Likar. This mobility was organized as a part of bilateral collaborative project “Grapevine, viruses and mycorrhizal fungi – analysis of the complex interplay”, led by Katarina Hančević, PhD, Croatian project coordinator, and Matevž Likar, PhD, Slovenian project coordinator. This bilateral project is funded through joined efforts from the Government of the Republic of Croatia and the Government of the Republic of Slovenia.