At the Institute for Sustainable Plant Protection, Emanuel is actively learning parts of the grapevine tissue culture protocol. Starting with familiarization with the protocols, laboratory equipment and the preparation of different types of nutrient media for stimulating the development of callus and embryos from the anthers and ovaries from grapevine inflorescence. In Turin, the correct method of sampling, storing and sterilizing of grapevine inflorescences has also been learned. From such inflorescences, a large number of anthers and ovaries will be isolated on a nutrient medium for callus induction.

Somatic embryogenesis is the process of developing embryos from cells that are not produced by the fusion of gametes, but have been differentiated into a somatic cells. In this case, in vitro development of an embryo from anthers/ovaries involves the dedifferentiation of the cell into a totipotent cell that gradually differentiates into callus tissue, and then an embryo from which a new plant develops.